In Vitro ADME

       The blood/plasma ratio assay can be used for pharmacokinetic studies in drug discovery and preclinical development. This assay helps to better understand the pharmacokinetic characteristics of drugs and can also provide reference for potential blood toxicity evaluations.  

       Blood Plasma Ratio (BP) is one of the important parameters for measuring the distribution of drugs from blood to tissues and organs, that is, the concentration ratio of compounds in blood and plasma. The experimental blood comes from humans, rats, mice, dogs, monkeys, etc.  

       In biological analysis, the concentration of the tested compound usually refers to the concentration in the plasma. For most compounds, there is no significant difference in their concentration in plasma compared to that in blood. However, it is possible that plasma concentrations may differ from blood concentrations because compounds may selectively bind proteins in blood cells. Therefore, testing the blood/plasma ratio of a compound can help to understand whether it enters and binds to proteins in blood cells. If the blood/plasma ratio is greater than 1, it indicates that the compound has entered the blood cells, and the PK parameters calculated using serum concentration will not reflect the PK properties of the compound well.