Microsome Stability TestThe stability analysis of liver microsomes is conducted by extracting microsomes from liver cells to study the metabolic stability of compounds. Liver microsomes are mainly the endoplasmic reticulum of cells and contain many enzymes involved in drug metabolism, including cytochrome P450, flavin monooxygenase, carboxylesterase and epoxide hydrolase. Since different batches of liver microsomes can vary significantly, it is necessary to plan ahead and order the same batch of liver microsomes if a rigorous comparison of liver microsomal stability is needed for compounds throughout the program.
| Study Protocol for Liver Microsomes Metabolic Stability | |
| Compound Test Concentration | 10 μM |
| Species of Microsomes | Rat, Mouse, Dog, Monkey, Human |
| Protein Concentration of Microsomes | 0.7 mg/mL |
| Incubation Time (min) | 0,10,30,60 |
| Co-factor | NADPH |
| DMSO Concentration in the System | <0.25% |
| Positive Control Compound | Clozapine, Verapamil NCF60 (incubate the system without NADPH for 60 minutes) |
| Detection Method | LC-MS/MS |
| Results | T1/2, CLint, CLH, ER |
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