Cell-based Assay

       Flow cytometry is widely used in fields of immunology, molecular biology, bacteriology, virology, cancer biology, and infectious disease. It can be used to characterize and analyze different cell populations, such as blood, bone marrow, lymph nodes, spleen, mucosal tissue and solid tumors. It can also be used for multi-omics analysis, such as simultaneous detection of gene and protein expression at the single-cell level.  

       The applications of flow cytometric analysis in cancer biology mainly include the following aspects: characterization and identification of cancer cells, such as detecting tumor markers, chromosome defects, cell cycle, and apoptosis; separation and enrichment of cancer cells, such as labeling and screening cancer cells using antibodies or fluorescent dyes; functional analyses of cancer cells, such as evaluating their proliferation, migration, invasion, drug resistance and metabolism; research on tumor microenvironment, such as analyzing immune cells, angiogenesis, cytokines, and immune checkpoints in tumors.  

       Flow cytometry is a technique that uses a flow cytometer to detect multiple characteristics of individual particles (single cell suspension, biological particles, etc.) or to sort specific populations. It can simultaneously analyze multiple parameters of a single cell, mainly used to measure the fluorescence intensity produced by fluorescent labeled antibody-detecting proteins, or ligands that bind to specific cell molecules, such as propidium iodide to DNA. It is fast, accurate and objective, and mainly includes techniques for liquid flow of samples, counting and sorting of cells, and computerized acquisition and analysis of data.  

       The technique of counting and sorting cells refers to the use of a flow cytometer to continuously analyze multiple parameters of a single cell flowing through an optical or electronic detector and to separate the cells according to their characteristics. There are two commonly used methods for cell sorting: flow cytometry and immunomagnetic cell sorting. Flow cytometric cell sorting is based on the detection of cell phenotypes by flow cytometry and uses charge sorting techniques to achieve the separation of cells of multiple specific phenotypes. Immunomagnetic cell sorting uses magnetic beads in combination with antibodies or ligands to enrich or remove cells of a specific phenotype through a magnetic field.