Cell-based Assay

       3D cell culture is an experimental technique commonly used in biological research which simulates the three-dimensional structure of cells in a physiological environment. Compared with traditional 2D cell culture, 3D cell culture is closer to the real environment of cell growth in the human body and therefore has higher biological significance.  

       In 3D cell culture, cells can form complex structures in three-dimensional space through self-assembly, scaffolds or microfluidics. These structures can be spheroids, tubular structures (tubules), films, etc. These structures can better simulate the morphology and physiological functions of tissues and organs, allowing researchers to better study biological issues, such as cancer development, cell proliferation, drug screening, etc.  

       In addition, 3D cell culture also provides better drug screening compared to 2D cell culture, as it can better predict the efficacy and toxicity of drugs in vivo, thus improving the efficiency of drug development.  

       In conclusion, 3D cell culture is an important experimental technique that can better simulate the growth and development of cells in physiological environments and has a wide range of applications.