Cell-based Assay

       High Content Screening (HCS) refers to the process of detecting the effects of screened samples on cell morphology, growth, differentiation, migration, apoptosis, metabolic pathways, and signal transduction while maintaining the integrity of cell structure and function. It involves obtaining a large amount of information related to genes, proteins, and other cellular components in a single experiment to determine their biological activity and potential toxicity. At the same time, it is also a high-resolution fluorescence digital imaging system aimed at obtaining multi-dimensional and real-time biological effect information generated by screened samples on cells, and a diversified and functional screening technology platform for detecting multiple indicators at the cellular level.  

       The advantage of high content screening is its ability to obtain quantitative information on multiple parameters in one experiment. It combines the detection technology of high-speed multi-channel imaging and powerful analysis software for multi-parameter analysis, which can quickly capture cellular, sub-cellular or tissue images and extract and analyze the image information. It overcomes the slow detection speed and complex operation of conventional imaging detection or flow-type multi-parameter detection, and achieves fast and high-quality detection of large sample quantities.  

       The traditional single-target research method is no longer suitable for the study of therapeutic drugs related to multi gene diseases and viral infections. However, high content screening (HCS) technology based on cells achieves simultaneous detection of multiple targets and parameters of compounds, allowing people to study the mechanism of action, metabolic pathways, and potential toxicity of drugs from the perspective of disease-related gene regulatory pathways and network levels. It also makes it possible to comprehensively evaluate the medicinal properties of active compounds at the cellular level, playing an increasingly important role in the development of new drugs.