Cell-based Assay

       Conducting cell proliferation assays can help researchers understand the effects of different factors on cell growth and division. It can be used to test the toxicity and growth inhibition effects of drugs, compounds, or other external factors, as well as to study the regulatory mechanisms of cell proliferation, cell cycle progression and gene expression.

       The cell proliferation assay is a common cell biology experiment used to study the proliferation ability of cells under different conditions. This assay usually assesses the ability of cells to proliferate by culturing them in media containing different concentrations of growth factors, drugs, or other stimuli and measuring changes in cell number.

       When performing cell proliferation experiments, it is necessary to first select appropriate cell lines, such as cancer cells, primary cells, etc. Then, the cells are inoculated in the culture medium containing the desired stimuli and the change in cell number is observed over a certain period of time (usually 24-72 hours). Finally, the ability of cell proliferation can be evaluated through cell counting, MTT reagent method, and cell proliferation curve.

       Cell proliferation assays can be used to evaluate the toxicity and anti-tumor activity of drugs, as well as to study the mechanisms of cell proliferation regulation, cell cycle progression and gene expression.