Cell-based Assay

       Cytokine analysis is used to study the expression and secretion of cytokines (including growth factors, cytokines, hormones, etc.). Cytokines are soluble proteins that can regulate biological processes such as cell proliferation, differentiation, apoptosis, and immune response.  

       In cytokine analysis, techniques such as enzyme-linked immunosorbent assay (ELISA), flow cytometry, and protein microarrays are commonly used to detect cytokine expression and secretion. Among them, ELISA is one of the most commonly used techniques, which can quantitatively determine the concentration of cytokines by binding specific antibodies to target cytokines.  

       Cytokine analysis can be used to study the biological functions of cytokines, diagnose diseases, and evaluate drug efficacy. For example, in cancer research, the expression level of tumor cytokines can be detected to evaluate the invasive and metastatic ability of tumors; in immunological research, the expression level of immune cytokines can be detected to evaluate the intensity and type of immune response.